Rearing and infection techniques for Amblyomma species to be used in heartwater transmission experiments.
نویسندگان
چکیده
Techniques for the rearing of Amblyomma species to be used in heartwater (Cowdria ruminantium) transmission experiments are discussed. These involve the breeding and maintenance of infected and non-infected strains of ticks. They include the feeding of ticks on sheep, rabbits, mice, tortoises and guinea fowl.
منابع مشابه
Detection of the agent of heartwater, Cowdria ruminantium, in Amblyomma ticks by PCR: validation and application of the assay to field ticks.
We have previously reported that the pCS20 PCR detection assay for Cowdria ruminantium, the causative agent of heartwater disease of ruminants, is more sensitive than xenodiagnosis and the pCS20 DNA probe for the detection of infection in the vector Amblyomma ticks. Here, we further assessed the reliability of the PCR assay and applied it to field ticks. The assay detected DNA of 37 isolates of...
متن کاملThe ecology of the African vectors of heartwater, with particular reference to Amblyomma hebraeum and Amblyomma variegatum.
The hosts, sites of attachment, life cycle, habitat requirements and seasonal abundance of Amblyomma astrion, Amblyomma cohaerens, Amblyomma gemma, Amblyomma hebraeum, Amblyomma lepidum, Amblyomma marmoreum, Amblyomma pomposum, Amblyomma sparsum, Amblyomma tholloni and Amblyomma variegatum, the 10 potential vectors of heartwater in Africa, are listed. Factors influencing the distribution and ab...
متن کاملDanger of introducing heartwater onto the American mainland: potential role of indigenous and exotic Amblyomma ticks.
The existence of heartwater on 3 islands of the Central Lesser Antilles and the presence of an efficient vector originating from Africa, Amblyomma variegatum, on most of the islands of this region constitute a serious threat for livestock on the American mainland. The disease can be introduced there either by infected animals or infected ticks. The most likely way is probably the transportation...
متن کاملHeartwater. The artificial transmission of Cowdria ruminantium in domestic ruminants and mice.
The artificial transmission of Cowdria ruminantium with infected blood, organ homogenates, peritoneal macrophages, tick stabilate and tissue culture cells is discussed. Organ homogenates prepared from the myocardium, spleen, kidneys and liver of diseased animals are commonly used to infect mice. The efficacy of organ homogenates as a source of C. ruminantium depends on factors such as the route...
متن کاملThe application of the indirect fluorescent antibody test in research on heartwater.
The preparation of the antigen, details of the reagents, the titration of the antispecies conjugates and the execution of the indirect fluorescent antibody test are described. The sensitivity and specificity of the test and its applicability to the detection of antibodies to Cowdria ruminantium are recorded. The test is both highly specific and sensitive and can be applied to a wide range of st...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Onderstepoort journal of veterinary research
دوره 54 3 شماره
صفحات -
تاریخ انتشار 1987